Evaluation of the immunigenicity and efficacy of two plasmid types encoding for PRRS virus GP4 (ORF4) and GP5 (ORF5) [Porcine Reproductive and Respiratory Syndrome]

Ferrari, M.; Gregori, A.; Corradi, A.; Villa, R.; Lombardi, G.; Borghetti, P.; Bottarelli, E.; Bonati, L.; Petrini, S.; Ramadori, G.; Amici, A.

Evaluation of the immunigenicity and efficacy of two plasmid types encoding for PRRS virus GP4 (ORF4) and GP5 (ORF5) [Porcine Reproductive and Respiratory Syndrome]

2006

إنجليزي. Control of PRRS infection can be carried out by using live-attenuated and inactivated vaccines marketed by different companies. However, they have been resulted capable of inducing an incomplete protection against the infection. The aim of this study was the evaluation of the potential use, as alternative strategy, of DNA plasmid vaccination (genetic vaccination). According to that, two different plasmids, derived from pVAX1 vector encoding the PRRSV ORFs 4 and 5, were tested. Afterwards, pig groups injected with the selected plasmids and the control groups were challenged with a virulent virus. The results show that the DNA plasmids didn't protect against the experimental infection with a virulent PRRSV strain and that only a cell mediate immune response was stimulated

اظهر المزيد [+]

إيطالي. Il controllo dell'infezione della PRRS può essere attuato mediante il ricorso a vaccini attenuati e inattivati commercialmente disponibili, ma risultati in grado di conferire una protezione incompleta nei confronti del virus virulento. Obiettivo dell'indagine è stata la valutazione della possibile applicazione, quale strategia alternativa, della vaccinazione con DNA plasmidico (vaccinazione genetica). A tal fine, la fase attuale ha riguardato la valutazione di due differenti plasmidi derivati dal vettore PVAX1 e codificanti ORF4 e ORF5 del virus della PRRS. Gruppi di suini inoculati con i plasmidi e corrispondenti soggetti di controllo sono stati successivamente infettati sperimentalmente con un ceppo di campo. I risultati hanno evidenziato che i plasmidi selezionati non sono stati in grado di proteggere dalla malattia e hanno stimolato soltanto una risposta immunologica di tipo cellulo-mediata

اظهر المزيد [+]

الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)

adn antigen antibody reactions blood serum body temperature cell mediated immunity cerdo control de enfermedades controllo delle malattie disease control dna elisa gene genes geni glicoproteine glycoproteins immune response immunità cellulo-mediata immunoglobuline immunoglobulins in vitro experimentation in vivo experimentation infection infezione inmunidad celular inmunoglobulina inoculation inoculazione interferon interferonas interferoni interferons interleuchine interleukine interleukins interleuquinas lelystad virus microbial proteins nucleotide sequence pcr plasmide plasmidi plasmidios plasmids porcin proteine microbiche prrs reazioni antigene-anticorpo risposta immunitaria sdrp sequenza nucleotidica siero del sangue sperimentazione in vitro sperimentazione in vivo srrp srrs suini swine temperatura corporea temperatura del cuerpo test elisa vaccination vaccinazione virus de lelystad virus di lelystad

المعلومات البيبليوغرافية

نُشرت في

Atti della Società Italiana di Patologia ed Allevamento dei Suini (Italy)

المجلد 32

ترقيم الصفحات

pp. 207-211

مواضيع أخرى

Immunite cellulaire; Glycoproteine; Reponse immunitaire; Temperature corporelle; Respuesta inmunologica; Experimentation in vitro; Reaccion antigeno anticuerpo; Inoculacion; Glicoproteinas; Vacunacion; Suero sanguineo; Experimentation in vivo; Serum sanguin; Proteine microbienne; Reaction antigene anticorps; Sequence nucleotidique; Infeccion; Controle de maladies; Experimentacion in vivo; Secuencia nucleotidica; Experimentacion in vitro; Proteinas microbianas

اللغة

إيطالي

ملاحظة

Summaries (En, It)

1 table

7 ref.

العنوان المترجم

Valutazione delle caratteristiche immunologiche ed efficacia protettiva di due tipi di plasmidi codificanti le glicoproteine GP4 (ORF4) e GP5 (ORF5) del virus PRRS [sindrome riproduttiva e respiratoria del suino]

النوع

Conference; Summary

مؤتمر المنظمة

[Italian Society for Swine Pathology and Breeding (SIPAS). Annual meeting], 32, Modena (Italy), 23-24 Mar 2006

في أجريس منذ: 2008-05-15

نوع الملف: AGRIS AP

مزود البيانات

تم تزويد هذا السجل من قبل Istituto di Servizi per il Mercato Agricolo Alimentare

اكتشف مجموعة مزود البيانات هذا في أجريس

تصفح الباحث العلمي من جوجل

إذا لاحظت أي معلومات غير صحيحة تتعلق بهذا السجل ، يرجى الاتصال بنا [email protected]

أجريس - النظام الدولي للعلوم الزراعية والتكنولوجيا

Share

Evaluation of the immunigenicity and efficacy of two plasmid types encoding for PRRS virus GP4 (ORF4) and GP5 (ORF5) [Porcine Reproductive and Respiratory Syndrome]

2006

الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)

المعلومات البيبليوغرافية