Single Cell Protein Production From Raw Starch In Fed_Batch Culture By Coculture Of Cryptococcus Aerius And Saccharomyces Cerevisiae
2008
Nahvi, I., Professor of Biotechnology, Department of Microbiology, Faculty of Science, Isfahan University, Isfahan, Iran. | Shafiei, R., M.Sc in Microbiology, Department of Microbiology, Faculty of Science, Isfahan University, Isfahan, Iran.
These days, with regard to food and industrial value of starch, wastewater containing starch is one of the main source of environmental contaminant in developing countries. In this research, after isolation and identification of an amylase producing yeast called Cryptococcus aerius which was capable of raw and soluble starch assimilation, single cell protein (SCP) production with three different culture patterns was investigated in lab fermenters with total volume of 7 l. In the first pattern, which was a pure-culture of C. aerius on raw wheat starch, final biomass and raw cell protein production was less than the second pattern of culture which was a coculure of C. aerius and S. cerevisiae. In the third culture pattern which was a modification of the second culture pattern, due to incomplete carbohydrate assimilation by yeasts, BOD5 decrease was not considerable. Finally it was proved that with the second culture pattern, 85- 95% of BOD5 was decreased (compared with the initial wastewater) and biomass with 40.2% (w/w) raw protein content could be achieved.
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