Expression and Characterization of a New Esterase Cloned Directly from Agrobacterium tumefaciens Genome
2006
Park, H.J. (The Catholic University of Korea, Bucheon, Republic of Korea) | Kim, Y.J. (The Catholic University of Korea, Bucheon, Republic of Korea) | Kim, H.K. (The Catholic University of Korea, Bucheon, Republic of Korea), E-mail: [email protected]
A new functional lipolytic enzyme (AT4) has recently been found from Agrobacterium tumefaciens C58 Cereon using a genome-wide approach. The enzyme has some sequence similarity to E. coli acetyl hydrolase, Emericella nidulans lipase, Moraxella sp. lipase, Acinetobacter lwoffii esterase, and Streptomyces hygroscopicus acetyl hydrolase. However, the sequence similarities are very low (less than 25%), suggesting that it is a new lipase/esterase enzyme. In the present study, intact cell of the A. tumefaciens strain was shown to have lipolytic activity on a tributyrin-LB plate.
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
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