Effects of Follicle Stimulating Hormone and Human Chorionic Gonadotrophin on the In Vitro Maturation of Canine Oocytes
2007
Kim, M.K. (Seoul National University, Seoul, Republic of Korea) | Oh, H.J. (Seoul National University, Seoul, Republic of Korea) | Jang, G. (Seoul National University, Seoul, Republic of Korea) | Hong, S.G. (Seoul National University, Seoul, Republic of Korea) | Park, J.E. (Seoul National University, Seoul, Republic of Korea) | Kim, H.J. (Seoul National University, Seoul, Republic of Korea) | Lee, H.S. (Woosong Information College, Daejeon, Republic of Korea) | Kim, S.C. (Shingu College, Seongnam, Republic of Korea) | Kang, S.K. (Seoul National University, Seoul, Republic of Korea) | Lee, B.C. (Seoul National University, Seoul, Republic of Korea), E-mail: [email protected]
The present study investigated the effects of follicle stimulating hormone (FSH) and human chorionic gona-dotrophin (hCG) on the nuclear maturation of canine oocytes. Oocytes were recovered from mongrel female ovaries in various reproductive states; follicular, luteal or anestrous stage. Oocytes were cultured in serum-free tissue culture medium (TCM)-199 supplemented with various concentrations of FSH (Exp. 1: 0, 0.5, 1.0 or 10 IU) or hCG (Exp. 2: 0, 0.5, 1.0 or 10 IU) or both (Exp. 3: 1 IU FSH + 1 IU hCG) for 72 hr to determine the effective concentration of these hormones, and to examine their combined effect. After maturation culture, oocytes were denuded in PBS containing 0.1% (w/v) hyaluronidase by gentle pipetting.
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