HSP70 level in the leaves of Phaseolus vulgaris intoxicated with cadmium
2007
Vasiljevic, Dj.,Institute for Biological Research Sinisa Stankovic, Belgrade (Serbia). Department of Biochemistry | Brkljacic, J.,Institute for Biological Research Sinisa Stankovic, Belgrade (Serbia). Department of Biochemistry | Papic, D.,Institute for Biological Research Sinisa Stankovic, Belgrade (Serbia). Department of Biochemistry | Vojnovic-Milutinovic, D.,Institute for Biological Research Sinisa Stankovic, Belgrade (Serbia). Department of Biochemistry | Dundjerski, J.,Institute for Biological Research Sinisa Stankovic, Belgrade (Serbia). Department of Biochemistry
In the present study, the expression of HSP70 in bean seedlings exposed for 5 or 15 days to increasing doses of cadmium was examined in order to learn more about the role of HSP70 in the ability of Phaseolus vulgaris to cope with chemical stress provoked by the toxic metal cadmium. Selected healthy beans were grown 20 days on plates with cotton soaked in Knop solution as a nutrient medium under optimal light and temperature conditions. Over the next 5 or 15 days, the formed seedlings were further watered with increasing doses of cadmium (1 microM - 100 microM Cd). Treatment was performed two times per day with 4 ml of CdCl2 solution. In conclusion, the level of HSP70 in kidney bean leaves depends on the amount of applied and accumulated cadmium, as well as on duration of the treatment. The basal level of constitutive HSP70 in untreated plants increased during growth. On the whole, the presented data show that both constitutive and inducible HSP70 isoforms play an important role in the respons to stress provoked by cadmium and imply the involvement of other protective mechanisms.
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