Insecticide resistance evaluation of the green leafhopper, Nephotettix cincticeps Uhler to the systemic insecticides by parafilm test method

Jirapong Jairin. Rice Research Institute. Ubon Ratchathani Rice Research Center; Kojima, Nobuki; Nagata, Toru. Faculty of Agriculture

Insecticide resistance evaluation of the green leafhopper, Nephotettix cincticeps Uhler to the systemic insecticides by parafilm test method

2003

Jirapong Jairin(Department of Agriculture, Bangkok (Thailand). Rice Research Institute. Ubon Ratchathani Rice Research Center) | Kojima, Nobuki(Tsukuba International Centre, Ibaraki (Japan)) | Nagata, Toru(Ibaraki University, Ibaraki (Japan). Faculty of Agriculture)

Insecticide resistance of the green leafhopper (GLH), Nephotettix cincticeps to systemic insecticides, imidacloprid, cartap, propaphos and disulfoton, was determined by a newly developed testing method, the parafilm test using an artificial membrane to feed the artificial diet composed of 2.5 percent sucrose and series dilution of insecticides. This test can measure both mortality (LC sub(50)) and feeding inhibition (Fl sub(50)). The three local populations of GLH with different resistance levels to organophosphates and carbamates were compared with LC sub(50) and Fl sub(50) obtained by this testing method. Feeding inhibition at sublethal doses was most remarkable, in imidacloprid followed by cartap with no regard to resistance levels, while feeding inhibition by propaphos and disulfoton was observed only with the resistant GLH.

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