The analysis of low molecular weight glutenin subunit gene sequences coding by Glu-A3 locus in 3 Xinjiang wheat landraces
2007
Zhang Qiqi, Ma Chuanxi, Si Hongqi
صينى. 麦谷蛋白可分为高分子量麦谷蛋白亚基（HMW-GS）和低分子量麦谷蛋白亚基（LMW-GS），其中低分子量麦谷蛋白亚基对小麦品质具有重要影响。本文采用PCR方法从中国小麦微核心种质中的3个新疆小麦品种红春麦（新疆玛纳斯）、红春麦（新疆昌吉）和红金包银（新疆伊吾）中分别得到了3个低分子量麦谷蛋白亚基（LMW-GS）新基因（GenBank．DQ519084、DQ519085和DQ517534）。它们具有LMW-i型基因的典型结构特征，与已报道的Glu-A3位点编码的LMW-GS基因序列有很高的一致性，高达79．06％～94．24％。DQ519084和DQ517534在C末端保守区有9个半胱氨酸残基，DQ519085其编码区内存在1个提前终止密码子，推测其为假基因。
اظهر المزيد [+] اقل [-]إنجليزي. Gluteinins are multimeric aggregates of high molecular weight glutenin subunit （HMW-GS） and low molecular weight glutenin subunit （LMW-GS）, which determined the quality in wheat. Using PCR-amplification method, three new LMW-GS genes （GenBank： DQ519084, DQ519085 and DQ517534） were isolated from genomic DNA of 3 Xinjiang wheat landraces （Hongchunmai from Xinjiang Changji, Hongchunmai from Xinjiang Manasi and Hongjinbaoyin from Xinjiang Yiwu）, which were parts of Chinese wheat mini-core collections. Nucleotide sequence comparison of them with other reported LMW-GS genes located at Glu-A3 loci showed the degree of identity among them ranged from 79.06% to 94.24%. The deduced amino acid sequences showed that they were typical LMW-i genes. The deduced sequence of DQ519084 and DQ517534 both contained 9 cysteine residues, one more cysteine residue in the C-terminal conserved domain than the typical LMW-GS genes. DQ519085 contained a stop code in its coding region, so it is presumed as a pseudogene.
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