Nucleolar vacuolation in soybean root meristematic cells during recovery after chilling
2008
Stepinski, D.,University of Lodz (Poland). Dept. of Cytophysiology
The nucleolar vacuole formation in soybean root meristematic cells from seedlings grown 3 d at temperature 25 deg C (control), 3 d at temperature 25 deg C and then transferred to 10 deg C (chilling) for 4 d, and after recovery for 1.5, 3, 6, 12 and 24 h at 25 deg C were observed on semi-thin sections. Simultaneously, autoradiographic studies with 3H-uridine on squashed preparations were carried out. During recovery of plants, the number of vacuolated nucleoli increased gradually from 24% after 1.5 h up to 40% after 24 h, while in the control there were 18% of nucleoli with vacuoles and after 4-d chilling only 5%. Labelling of cells during 20-min incubation in 3H-uridine and during 80-min post-incubation in non-radioactive medium was increased in recovered plants in comparison with the control and chilled plants. In conclusion, nucleolar vacuoles in soybean plants are formed as a result of migration of granular component accumulated in nucleolus during 4-d chilling.
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