Developmental expression of beta-glucosidase in olive leaves
2009
Wang, W.,Henan Agricultural Univ., Zhengzhou (China). Coll. of Life Science | Li, C.Q.,Henan Agricultural Univ., Zhengzhou (China). Coll. of Life Science | Hu, X.L.,Henan Agricultural Univ., Zhengzhou (China). Coll. of Life Science
Plant beta-glucosidases catalyze the hydrolysis of glycosidic linkages and play a vital role in defense against pathogens and stress. The present work investigated the relationship between leaf development and beta-glucosidase protein content in Olea europea (cv. Picual) leaves. The total chlorophyll content increased with leaf age in current-season leaves. Immunoblot analysis revealed that the content of 61 kD protein of beta-glucosidase also increased with leaf age, and that the enzyme existed in three isoforms (pI 5.8-6.2). Statistical analysis indicated a strong correlation between chlorophyll and beta-glucosidase protein contents.
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