Increased cold tolerance in Arabidopsis thaliana transformed with Choristoneura fumiferana glutathione S-transferase gene
2009
Huang, C.,South China Normal Univ., Guangzhou (China). Guangdong Key Lab. of Biotechnology for Plant Development | Guo, T.,South China Normal Univ., Guangzhou (China). Guangdong Key Lab. of Biotechnology for Plant Development | Zheng, S.C.,South China Normal Univ., Guangzhou (China). Guangdong Key Lab. of Biotechnology for Plant Development | Feng, Q.L.,South China Normal Univ., Guangzhou (China). Guangdong Key Lab. of Biotechnology for Plant Development | Liang, J.H.,South China Normal Univ., Guangzhou (China). Guangdong Key Lab. of Biotechnology for Plant Development | Li, L.,South China Normal Univ., Guangzhou (China). Guangdong Key Lab. of Biotechnology for Plant Development
A glutathione S-transferase (GST) gene cloned from the lepidopteran spruce budworm, Choristoneura fumiferana, was transformed into the model plant Arabidopsis thaliana. The CfGST-transgenic and wild-type A. thaliana were subjected to 4 and 10 deg C for 48 h and their cold resistance was studied. The GST activity of the transgenic plants was 46.6 and 35.7% higher than that of the wild-type plants after 48 h under 4 and 10 deg C, respectively. Relative membrane permeability and malondialdehyde content in the transgenic plants were lower while contents of the chlorophyll and proline were higher than those in the wild-type plants under 4 and 10 deg C. The survival rate of the transgenic plants was 43.7% for 24 h under 0 deg C, while survival rate of wild-type plants was 28.3%. The results indicated that the insect GST could enhance cold resistance in the transgenic A. thaliana.
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