In-vitro genotoxicity of some phenoxy compounds using multiple genetic assay system on some selected cultured cells of common catfishes (heteropneustes fossilis and clarias batrachus).
2004
Faridi, W.A., Chemical Mutagenesis Laboratory, Aligarh (India)
Fish are useful for aquatic genotoxicity testing and they provide an excellent source of material for the study of mutagens and carcinogenic potential of water pollutants since they are quatic vertebrate organisms that can metabolise, concentrates and store waterborne pollutants. The parameters used in the project are found very effective and appropriate for genotoxicity testing especially the latest techniques like comet assay and binucleated blocked erythrocytes method for micronucleus test (MNT) produced excellent results. Cytogentic methods are probably the most sensitive and efficient way to detect the effects of genotoxins. After the initial screening of large number of cells, a rise in frequency of aberrant metaphase were observed in all exposure groups irrespective of the compounds used, though variations at chemicals and exposures levels does exist. The cultured cells of Heteropneustes fossilis and Clarias batrachus tend to be very sensitive to phenolic compounds and responded favourably to even initial doses. The use of fish cell cultures for genotoxicity testing was found very important for determining the initial effects of various phenoxy compunds to the catfishes. Some natural substance like neem (leaves extracts) was observed to impart protective effect to the gentic elements and were active against well known mutagens. The overall results of the project indicates that the chlorophenolic compounds tested in this project (PCP, 2, 4-D and butachlor) contain agents capable of inducing DNA damage in the catfish Clarias batrachus and Heteropneustes fossilis.
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
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تم تزويد هذا السجل من قبل Indian Council of Agricultural Research