A DIAGNOSTIC MULTIPLEX POLYMERASE CHAIN REACTION ASSAY FOR OCULAR HERPETIC INFECTIONS
2004
Kargar, R., Razi Vaccine&Serum Research institute, Tehran, Iran | Sharifzad, F., Razi Vaccine&Serum Research institute, Tehran, Iran | Behzad-Behbahani, A., Shiraz University of Medical Scienses, Shiraz, Iran | Shahrabadi, M., Iran University of Medical Scienses, Tehran, Iran | Mojgani, N., Razi Vaccine&Serum Research institute, Tehran, Iran
A multiplex PCR (m-PCR) technique was used to diagnose and differentiate ocular herpes virus infections including Varicella zoster virus (VZV) and herpes simplex virus (HSV) types 1 and 2. Conjunctival swab, corneal scraping, and ocular fluid samples were collected from 28 patients. Using a specific pair of primer, for thymidin kinase gene, both HSV- 1 and HSV-2 DNAs were amplified. A set of primer flanking a 208bp of the DNA-polymerase gene was also used to amplify VZV DNA. The sensitivity of the m-PCR for detection of HSV and VZV in clinical samples was 80.9% and 95%, respectively. Parts of the specimens were cultured on Vera, Hep II, and MRC-5 cell lines. The sensitivity of the cell culture for isolation of HSV and VZV was 62.9% and 72.7%, respectively. Using statistical analysis of the results a significant difference (P=0.001) between virus isolation and m-PCR for detection of HSV and VZV in clinical specimen was noticed. Both HSV and VZV DNAs were detected in 1 out of 28 (3.5%) specimens exclusively by m-PCR. Results indicate that m-PCR is a more reliable method in rapid diagnosis of herpes viruses DNA in clinical samples than virus isolation.
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
المعلومات البيبليوغرافية
تم تزويد هذا السجل من قبل Agricultural Research and Education Organization