Effect on growth environment of isoorientin content of sasa leaf (Sasa senanensis Rehder)
2008
Matsuta, T.(Tokyo Univ. of Agriculture, Abashiri, Hokkaido (Japan). Faculty of Bio-Industry) | Yamazaki, M. | Tomizuka, N. | Nishizawa, M. | Suzuki, T.
Sasa leaves growing wild in east area of Hokkaido, Japan, and soil were collected a two month from May to Nov. of 2005. Isoorientin was extracted with MeOH from sasa leaf, and prepared with liquid-liquid distribution and then column chromatography. The quantitative assay of isoorientin was used with LC/MS. Soil constituents were determined about mineral elements (Na, Mg, Si, P, Ca, Zn, B) and the nitrogen in the form of nitrate (NO3sup(-)) and soluble N applied as ammonium (NH4sup(+)). Isoorientin content of sasa leaves were correlated with phosphorous (r=-0.540 P0.01) in lower layer soil (depth 20-30cm). However, there was no correlation between isoorientin content and other constituents in soil. Isoorientin content of sasa leaf grown in open field was higher one grown in forest floor, was high in May and Nov., and the tendency to decrease in July to Sep. Isoorientin content of Kumai sasa (Sasa senanensis REHDER) was lowest during sasa genus grown in Hokkaido. Isoorientin content of sasa leaf growing in snow, at relative light intensity 0%, was ca. 30 (microg/g dry matter), and increased to ca. 200 (microg/g dry matter) for 2 weeks after removal snow from leaf in Apr. of 2006. Sasa leaf grown above snow, at relative light intensity (rPPFD) 35%, contained ca 310 (microg/g dry matter) of isoorientin. Therefore, the habitat isolation may have been caused by the difference of isoorientin content of sasa leaf.
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