Perchloric acid-soluble protein binds hypoxhantine phosphoribosyl transferase, but not affect its enzymatic activity
2008
Kanouchi, H.(Kagoshima Univ. (Japan)) | Tone, S. | Okabe, M. | Matsumoto, M. | Oka, T.
Perchloric acid-soluble protein (PSP) is a highly conserved protein in the course of evolution. There was no report about PSP associated protein, although various functions of PSP have been reported. We previously revealed a candidate gene for PSP binding protein, hypoxanthine-guanine phosphoribosyltransferase (HPRT) was identified by two hybrid screening systems. Using glutathione S-transferase (GST)-pull down method and immunoprecipitation method, we concluded that PSP and HPRT could directly bind. We examined whether PSP affected HPRT enzymatic activity in vitro or not. As a result, PSP showed no effect on its activity. The meaning of PSP and HPRT binding has not been addressed.
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