Identification of cDNAs encoding the WD repeat protein and auxin-regulated protein in Nicotiana glutinosa leaves induced in response to tobacco mosaic virus-infection at 25 deg C
2008
Abe, M.(Kyushu Univ., Fukuoka (Japan)) | Hayashi, T. | Hada, K. | Kimura, M.
Nicotiana glutinosa represents resistance to tobacco mosaic virus (TMV)-infection by causing a localized hypersensitive response (HR) at 25degC. The fluorescent differential display (FDD) analysis followed by reverse transcription (RT)-PCR indicated that two transcripts encoding a WD-repeated protein and a putative auxin-regulated protein were significantly increased upon TMV-infection at 25degC. The two full-length cDNAs (Ng0881 and Ng1851), which encode the WD repeat protein and auxin-regulated protein, were cloned, and the complete nucleotide sequences were determined. The deduced amino acid sequence for Ng0881 has seven repetitive WD repeats, sharing 81% identical residues with the homologue from Oryza sativa, while that for Ng1851 shares 68% identical residues with the auxin-regulated protein from Oryza sativa. It could be speculated that translational products of the genes ng0881 and ng1851 may serve as signal mediators required for TMV-resistance.
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