Characterization of lumen proteins from the labial glands of 5th instar larvae of Agrius convolvuli (Lepidoptera: Sphingidae)
2009
Zha, X.(Shinshu Univ., Ueda, Nagano (Japan). Faculty of Textile Science and Technology) | Shirai, K. | Li, J. | Chen, K. | Kanekatsu, R. | Kiguchi, K.
Larval labial glands of the sweet potato hornworm, Agrius convolvuli, secrete feeding- and wandering-stage specific proteins, which differ depending on the stage of development. The purpose of this study was to clarify the characteristics of feeding-stage specific proteins acquired from feeding-stage larvae. Using two-dimensional (2D) gel electrophoresis, a total of 11 protein spots were detected in proteins collected from the labial gland lumens of 5th instar day 2 larvae. N-terminal amino acid sequencing revealed that the 67, 70 and 74 kDa proteins had similar sequences, and were Asn and Ala rich. The 33 and 34 kDa proteins were also similar, with both characterized as having characteristic Gly-X repeats. In addition, a number of similar mass (m/z) peaks were detected in the 67, 70 and 74 kDa proteins and 33 and 34 kDa proteins by use of MS spectrometry. These results suggest that some of the feeding-stage specific proteins are derived from homologous genes have similar functions.
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