Antimutagenic property of Lactococcus lactis subsp lactis J525-2
2009
Tsuda, H.(Okayama Univ. (Japan)) | Ejima, Y. | Shuangquan. | Miyamoto, T.
The purpose of this study was to evaluate a factor of an antimutagenic activity of Lactococcus lactis subsp. lactis J525-2. A fermented milk prepared with this strain showed antimutagenic activity against food-borne mutagens such as 3-amino-1,4-dimethyl-5H-pyrido indole (Trp-P-1), 2-amino-6-methyldipyrido imidazole (Glu-P-1), and 2-amino-3,4-dimethyl-imidazo quinoline (MeIQ). Cultures of strain J525-2 showed high antimutagenic activity, but cell free culture showed little activity. These results suggest that extracellular products of strain J525-2 have no antimutagenic activity, and the cells play major part of the antimutagenic activity. The binding of Trp-P-1 to cells of strain J525-2 was confirmed. Mutagen binding occurred instantaneously and reached maximum at 10 min incubation. Prolongation of incubation time (up to 50 min) had no influence on the binding. The binding ability was strongly affected by pH, and the highest percentage binding was noted at pH 6.0. Cell of Lactococcus lactis subsp. lactis J525-2 was suggested to play a major role in the antimutagenic activity, and the cell could bind food-borne mutagens.
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