Antimicrobial resistance and genetic characteristics of Salmonella typhimurium isolated from horses in Hokkaido, Japan
2009
Niwa, H.(Japan Racing Association, Shimotuske, Tochigi. Equine Research Inst.) | Anzai, T. | Izumiya, H. | Morita Ishihara, T. | Watanabe, H. | Uchida, I. | Tozaki, T. | Hobo, S.
In this study, we examined the antimicrobial susceptibility of 16 Salmonella typhimurium isolates obtained from horses, and applied several genetic methods, namely polymerase chain reaction (PCR) for detecting class 1 integrons, multiplex PCR for detecting multidrug resistant S. typhimurium definitive phage type 104 (MR-DT104), and fluorescent amplified-fragment length polymorphism (FAFLP). Seven isolates with an ampicillin, chloramphenicol, streptomycin, sulfonamide, and tetracycline (ACSSuT) type resistance pattern, harbored two class 1 integrons with sizes of 1.2 and 1.0 kb, and were identified as DT104 by bacteriophage typing. These isolates also showed a typical MR-DT104 amplification pattern, which was positive for flosub(st), spvC, invA and int, in multiplex PCR. In the FAFLP analysis, the equine DT104 isolates and the previously reported ACSSuT-type resistant bovine isolates, which were also isolated in Hokkaido were included in the same genetic cluster. Our results retrospectively indicate that MR-DT104 infection has existed in horses in Japan at least since 1996, and it was suggested that there is a highly epidemiological relationship between the equine MRDT104 isolates and certain multidrug resistant bovine isolates in the same area.
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