Overexpression of Arabidopsis Homogentisate Phytyltransferase or Tocopherol Cyclase Elevates Vitamin E Content by Increasing γ-tocopherol Level in Lettuce (Lactuca sativa L.)
2007
Lee, K.E. (Seoul National University, Seoul, Republic of Korea) | Lee, S.M. (Seoul National University, Seoul, Republic of Korea) | Park, S.R. (Seoul National University, Seoul, Republic of Korea) | Jung, J.W. (Seoul National University, Seoul, Republic of Korea) | Moon, J.K. (Seoul National University, Seoul, Republic of Korea) | Cheong, J.J. (Seoul National University, Seoul, Republic of Korea) | Kim, M.K. (Seoul National University, Seoul, Republic of Korea), E-mail: [email protected]
Tocopherols, essential components of the human diet, are synthesized exclusively by photosynthetic organisms. To increase tocopherol content by increasing total flux to the tocopherol biosynthetic pathway, genes encoding Arabidopsis homogentisate phytyltransferase (HPT/VTE2) and tocopherol cyclase (TC/VTE1) were constitutively overexpressed in lettuce (Lactuca sativa L.). Total tocopherol content of the transgenic plants overexpressing either of the genes was increased by more than 2-fold mainly due to an increase in γ-tocopherol. However, chlorophyll content in the HPT/VTE2 and TC/VTE1 transgenic lines decreased by up to 20% and increased by up to 35%, respectively (P less than 0.01). These results demonstrate that manipulation of the tocopherol biosynthetic pathway can increase or decrease chlorophyll content depending on the gene introduced.
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