Effect of Plant Growth Regulators on Callus Induction and Plant Regeneration of Perennial Ryegrass (Lolium perenne L.)
2007
Lee, K.W. (Gyeongsang National University, Jinju, Republic of Korea) | Lee, D.G. (Gyeongsang National University, Jinju, Republic of Korea) | Ahsan, Nagib (Gyeongsang National University, Jinju, Republic of Korea) | Won, S.H. (Gyeongsang National University, Jinju, Republic of Korea) | Lee, S.H. (National Institute of Animal Science, RDA, Suwon, Republic of Korea) | Kim, K.Y. (National Institute of Animal Science, RDA, Suwon, Republic of Korea) | Choi, G.J. (National Institute of Animal Science, RDA, Suwon, Republic of Korea) | Seo, S. (National Institute of Animal Science, RDA, Suwon, Republic of Korea) | Lee, B.H. (Gyeongsang National University, Jinju, Republic of Korea), E-mail: [email protected]
Optimum tissue culture conditions for an efficient induction of embryogenic callus from mature seeds of perennial ryegrass (Lolium perenne L.) and regeneration of plants from callus tissues were investigated. MS medium containing 3 mg/L 2,4-D and 0.1 mg/L BA was optimal for embryogenic callus induction from mature seeds. The highest plant regeneration frequency (58.3%) was observed when the embryogenic callus tissues were cultured on N6 medium supplemented with 1 mg/L 2,4-D and 3 mg/L BA. Regenerated plants were grown normally when shoots transplanted to the soil.
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