Aeration volume and photosynthetic photon flux affect cell growth and secondary metabolite contents in bioreactor cultures of Morinda citrifolia
2008
Ahmed, Saifullah (Chungbuk National University, Cheongju, Republic of Korea) | Hahn, E.J. (Chungbuk National University, Cheongju, Republic of Korea) | Paek, K.Y. (Chungbuk National University, Cheongju, Republic of Korea), E-mail: paekky@chungbuk.ac.kr
To improve their growth and secondary metabolite production, we cultured Morinda citrifolia leaf cells for 3 weeks in bioreactors with different aeration volumes (0.05, 0.1, 0.2, or 0.3 vvm; or 0.05/0.1/0.2/0.3 vvm, as increased at 5-d interval), and photosynthetic photon fluxes (PPF; 0, 15, 30, or 45 μmoL m-² s-¹). Cell growth was greatest (15.6 g L-¹ dry weight) at 0.3 vvm whereas the accumulation of secondary metabolites (total anthraquinones, phenolics, and flavonoids) was maximized at 0.1 vvm. A PPF of 15 μmoL m-² s-¹ accelerated the accumulation of both cell biomass and metabolites. Dark-culturing suppressed cell growth, while a high PPF (45 μmoL m-² s-¹) inhibited metabolite biosynthesis. Further studies are required to understand the reason for differences in the effect of light on cell growth and secondary metabolite contents in M. citrifolia cell cultures.
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