Microsatellite and single nucleotide polymorphism of prolactin gene (PRL) in Latvian brown cattle breed
2008
Sjakste, T., University of Latvia, Salaspils (Latvia). Inst. of Biology. Genomics and Bioinformatics Research Group | Jemeljanovs, A., Latvia Univ. of Agriculture, Sigulda (Latvia). Agency Research Inst. of Biotechnology and Veterinary Medicine Sigra | Zitare, I., Latvia Univ. of Agriculture, Sigulda (Latvia). Agency Research Inst. of Biotechnology and Veterinary Medicine Sigra | Paramonova, N., University of Latvia, Salaspils (Latvia). Inst. of Biology. Genomics and Bioinformatics Research Group | Trapina, I., University of Latvia, Salaspils (Latvia). Inst. of Biology. Genomics and Bioinformatics Research Group | Sterna, V., Latvia Univ. of Agriculture, Sigulda (Latvia). Agency Research Inst. of Biotechnology and Veterinary Medicine Sigra | Paura, L., Latvia Univ. of Agriculture, Jelgava (Latvia). Faculty of Information Technologies
Prolactin is a polypeptide hormone with multiple functions including regulation of mammary gland development, milk secretion, and expression of milk protein genes. Previously it was reported that single nucleotide polymorphism in prolactin gene (PRL) is associated with milk yield and fat content in Black-and-white cows. The aim of our study was to genotype two PRL gene polymorphisms including microsatellite (MS) in promoter region and cSNPA**7496→G in exon IV in 100 animals of the Latvian Brown (LB) cattle breed of 6 herds and to study polymorphism association with milk performance traits. In all the herds MS locus was presented by a common allele of 160 bp and a rare one of 156 bp (corresponding frequencies were 0.86 and 0.14) with genotypes of 160/160 (73%) and 156/160 (27%). Allele 156 bp was not revealed in homozygous state in the analyzed herds. Animals with genotype 160/160 were characterized with the highest milk yield, fat and protein content. Alleles G**7496 and A**7496 were revealed as common and rare cSNP in exon IV (frequencies 0.93 and 0.07 correspondingly). Homozygotes G**7496/G**7496 were predominant in all herds (86%) compared to heterozygotes G**7496/A**7496 (13%). Only one homozygote of the rare allele A**7496 was revealed. It was found that heterozygotes G**7496/A**7496 produced higher milk yield, but genotype G**7496/G**7496 was associated with higher fat and protein content. Results obtained in this study confirmed potential usefulness of PRL gene polymorphisms as molecular markers for genetic improvement of milk performance traits in cattle.
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