Hordein diversity in spring barley genotypes related to crude protein content | Hordeīna daudzveidības izvērtējums vasaras miežu genotipiem saistībā ar kopproteīna saturu graudos
2009
Bleidere, M., State Stende Inst. of Cereal Breeding, Dizstende, Talsu reg. (Latvia) | Grunte, I., State Stende Inst. of Cereal Breeding, Dizstende, Talsu reg. (Latvia)
The aim of the study was to assess the diversity of hordein banding patterns in different spring barley genotypes (covered, hull-less, two-row, and six-row) and to identify the relation of hordein patterns and hordein polypeptide bands with total protein content. The study was carried out at the State Stende Cereal Breeding Institute from 2004 to 2006. On the basis of sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE), 5 D hordein, 16 C hordein, and 28 B hordein banding patterns were detected. In total, 26 hordein polypeptide bands were recognized. Cluster analysis based on hordein patterns classified spring barley genotypes into three groups, and two of them significantly differed in crude protein content. Association was found between definite hordein patterns and crude protein content. There were found five covered varieties with identical hordein banding patterns but with varied crude protein content (111.9 to 154.3 g kgE-1). The patterns for these genotypes were distinctly different in the intensity and density of the colour of hordein polypeptide bands, especially in C hordein. Among all the genotypes screened, 10 hordein polypeptide bands revealed significance in identifying of genotypes with definite crude protein.
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