Partitioning of acid protease from Penicillium roqueforti in aqueous two-phase system polyethylene glicol/phosphate
2008
Pericin, D.M., University of Novi Sad, Novi Sad (Serbia). Faculty of Technology | Madjarev-Popovic, S.Z., University of Novi Sad, Novi Sad (Serbia). Faculty of Technology | Vastag, Z.G., University of Novi Sad, Novi Sad (Serbia). Faculty of Technology
From the results obtained in this study, it can be concluded that the partitioning of acid protease in aqueous two-phase system composed of polyethylene glycol and sodium phosphate indicate the applicability of ATPS for partial enzyme recovery from the top phase. Furthermore, when the completely randomized factorial design was used to optimize of separation factor the obtained results were similar to experimentally ones, indicating the possible application of factorial design onto ATPS partition and purification.
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