Clonal fidelity of chrysanthemum regenerated from long term cultures
2006
Jevremovic, S. | Trifunovic, M. | Nikolic, M. | Subotic, A. | Radojevic, Lj., Institute for Biological Research Sinisa Stankovic, Belgrade (Serbia)
Morphological characteristics of flowers of long term regenerated chrysanthemum, cv. White Spider, after ten years of micropropagation are investigated. Shoot cultures are established and maintained more than ten years by stem segment culture on MS medium supplemented with BAP and NAA (1.0, 0.1 mg/L, respectively). Rooting of shoots (100%) has done on MS medium without hormones and it was very successful after ten years, as well as, two or eight years of micropropagation. Acclimation of rooted chrysanthemum plantlets at greenhouse conditions was excellent and after appropriate photoperiod in vitro plants flowered 90.3% and have the same flower color, shape and size as mother plants. Flower color changes of in vitro plants are observed during another flowering cycle one year after acclimatization. Observed variations of chrysanthemum flowers could be attributed to epigenetic factors.
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