The regeneration ability of raspberry and blackberry samples was studied in vitro
2008
Lupysheva, Yu.V. | Dunaeva, S.E. | Pendinen, G.I. | Novikov, L.Yu. | Saveljeva, N.V., St. Petersburg State Univ. | Lutova, L.A. | Gavrilenko, T.A.
The varieties of raspberry (6 ones) and blackberry (6 ones) recommended for the North-Western part of Russia have been explored. The samples belong to the in vitro collection of the N.I. Vavilov All-Russia Research and Development Institute of Plant Growing. The more efficient nutrient medium type (MS + 2 mg/1 BAP + 0,1 mg/l IBA) and the explant type (segments of the leaves and leaf petioles) have been determined. There was no regeneration from root explants and at the usage of modified media (MS + TDZ). The genotypes with a relatively high regeneration ability were selected (Young - 22.4%; Ashton Cross - 13.5%; Kaskad - 13.8%). The adventive regenerants had normal morphology and a stable chromosome number. The PCR method has been planned to use for determining genetic stability of the regenerated plants. The transformants of blackberry cv. Young with the marker gene np2 (the one of resistance to kanamycine) and interferon target gene of a bull JFN6 were obtained. The transformant selection was done using the selective medium with kanamycine. Their transgenic character of experimental plants was proved by PCR analysis using a primer to interferon gene
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