Application of a simple and rapid pre-treatment procedure in the high performance liquid chromatographic analysis of deoxynivalenol and zearalenone in beer
2008
Belajová, E., Food Research Institute, Bratislava (Slovak Republic) | Rauová, D., Food Research Institute, Bratislava (Slovak Republic)
In this study, newly developed analytical methods for determination of deoxynivalenol (DON) and zearalenone (ZEA) in beer are described. Four processes for beer pre-treatment prior to HPLC analysis of both mycotoxins using diode-array (for DON), and fluorescent detection (for ZEA) were examined. Easy-to-use procedures of beer refining using specific immunoaffinity columns (IAC) Donprep and Easi-Extract Zearalenone (both from R-Biopharm Rhône, Glasgow, United Kingdom) were designed for DON and ZEA isolation, respectively. Maximum capacities of both tested IAC were explored in order to minimize the risk of their overloading with the beer sample. The minimal detectable amount was 6 micro g per l for DON and 4 micro g per l for ZEA. In a random survey, fourteen commercial beer samples were investigated for DON and ZEA by these methods. While ZEA was not detected in any tested sample, trace concentrations of DON (6–7 micro g per l) were found in 29% of the analysed beer samples. Concentrations higher than 7 micro g per l were detected in 28% of beer samples. The highest concentration of DON detected by this method in a lager beer was 33.2 micro g per l.
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