Effect of biopsy and freezing methods on quality of dairy cattle embryos | เธเธฅเธเธญเธเธเธฒเธฃเธเธฑเธเนเธเนเธเนเธฅเธฐเธงเธดเธเธตเนเธเนเนเธเนเธเธเนเธญเธเธธเธเธ เธฒเธเธเธฑเธงเธญเนเธญเธเนเธเธเธก
2008
Dumrongrak Rakwongrit(Khon Kaen University, Khon Kaen (Thailand). Faculty of Agriculture. Department of Animal Science) E-mail:[email protected] | Thevin Vongpralub(Khon Kaen University, Khon Kaen (Thailand). Faculty of Agriculture. Department of Animal Science) E-mail:[email protected] | Yupin Phasuk(Khon Kaen University, Khon Kaen (Thailand). Faculty of Agriculture. Department of Animal Science)
Aim of this study was to compare the survival rates of intact embryos and biopsied embryos cryopreserved by slow freezing and vitrification in direct transfer medium. Blastocyst embryos of superovulated lactating hybrid Holstein Friesian cows (GT 87.5 percent) were used in this study. Embryos at grades 1-2 (n= 83) were randomly assigned into 4 groups (groups1: intact embryos slow freezing n=20, groups2: biopsied embryos slow freezing n=21, groups3: intact embryos vitrification n=21 and groups4: biopsied embryos vitrification n=21). Post-thaw quality of grade 1-2 embryos in groups 1-4 were 80.00 percent, 61.90 percent, 52.38 percent and 33.33 percent respectively (P LT 0.05). After 12 h of co-culture with bovine oviductal epithelial cells, the survival rate were 85.00 percent, 47.61 percent, 52.38 percent and 33.33 percent respectively (P LT 0.01). The present study indicated that survival rates of biopsied embryos cryopreserved by slow freezing was higher than that of biopsied embryos cryopreserved by vitrification.
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