Effects of Aquanin plus (beta-cyclodextrin cysteamine hydrochloride) on the growth, survival and immune characteristics of postlarvae Pacific white shrimp (Litopenaeus vannamei) | ผลของสาร Aquanin plus (beta-cyclodextrin cysteamine hydrochloride) ต่อการเจริญเติบโต อัตรารอด และระบบภูมคุ้มกันของกุ้งขาวแวนนาไม ระยะโพสลาร์วา (Litopenaeus vannamei)

Effects of Aquanin plus (beta-cyclodextrin cysteamine hydrochloride) on the growth, survival and immune response in postlarvae (PL) Pacific white shrimp (Litopenaeus vannamei) was conducted under laboratory conditions. For the determination of the growth-promoting and immunostimulant effects of Aquanin plus administration in the diet, tests were carried out in three treatments (with three replicates per treatment). Each replicate consisted of 50 shrimp (PL20) in 500-liter tanks. Shrimp were fed four times daily for 60 days with pelleted feed containing graded levels of Aquanin plus (0 percent, 0.05 percent and 0.1 percent of the feed). After 60 days of dietary administration, shrimp fed with 0.1 percent Aquanin plus had an average body weight (4.43+-1.17 g) significantly higher (P LT 0.05) than that of 0.05 percent Aquanin plus (3.83+-1.29 g) and control groups (3.60+-1.38 g). Percentage survival of shrimp fed with 0.1 percent Aquanin plus (88.89+2.55 percent) and 0.05 percent Aquanin plus (85.00+-1.67 percent) were significantly higher (P LT 0.05) than the control (70.56+-4.19 percent). The shrimp immune responses were measured by total hemocyte count (THC), phenoloxidase activity and superoxide dismutase. The results showed that shrimp which consumed diets containing 0.1 percent Aquanin plus had significantly higher (P LT 0.05) THC, phenoloxidase activity and superoxide dismutase than control group, but was not significantly different from shrimp fed with Aquanin plus 0.05 percent. After intramuscular injection of Vibrio harveyi at 2.55*10**(5) CFU/ml (24-h LD50 level) to shrimp fed Aquanin plus for 60 days, shrimp fed with 0.1 percent Aquanin plus had survival rate (73.33+-5.77 percent) significantly higher (P LT 0.05) than shrimp fed with 0.05 percent Aquanin plus (56.67+-5.77 percent) and control groups (43.33+-5.77 percent). The present study indicated that oral administration of 0.1 percent Aquanin plus could increase the growth, survival and immune response of postlarvae L. vannamei.