Effects of ultrasound/dilute H2SO4 pretreatment on cellulase activity of corn straw liquid fermentation | 超声波/稀H2SO4预处理对玉米秸秆液体发酵产纤维素酶的影响
2009
Yang Peizhou, Hefei University of Technology, Hefei (China), School of Biotechnology and Food Engineering | Jiang Shaotong, Hefei University of Technology, Hefei (China), School of Biotechnology and Food Engineering | Pan Lijun, Hefei University of Technology, Hefei (China), School of Biotechnology and Food Engineering
[Objective] The aim was to study pretreatment of ultrasound enhancing dilute H2SO4 on cellulase activity of corn straw liquid fermentation and explore the pretretment’ s optimal conditions. [Method] By using orthogonal test, the pretretment of ultrasound enhancing dilute H2SO4 on corn straw was studied, then straw was fermented as the sole carbon source. Finally, the cellulase activity in extracellular fermentation broth was determined. [Result] The results showed that cellulase activity in extracellular broth was greatest under the conditions of acid bath time 3 h, acid concentration 3.5%, ultrasonic power 150 W, and ultrasonic time 5 h. They were FPA 15.82 U/ml, Cx 39.9 U/ml, β-Glu 55.94 U/ml respectively. [Conclusion] Under the above conditions, extracellular cellulase production has a high stability.
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