Barley and malt polyphenols and their antioxidant properties
2010
Dvorakova, M.,Vysoka Skola Chemicko-technologicka, Prague (Czech Republic). Ustav Kvasne Chemie a Bioinzenyrstvi | Dostalek, P.,Vysoka Skola Chemicko-technologicka, Prague (Czech Republic). Ustav Kvasne Chemie a Bioinzenyrstvi | Skulilova, Z.,Vysoka Skola Chemicko-technologicka, Prague (Czech Republic). Ustav Kvasne Chemie a Bioinzenyrstvi | Jurkova, M.,Vyzkumny Ustav Pivovarsky a Sladarsky, Prague (Czech Republic) | Kellner, V.,Vyzkumny Ustav Pivovarsky a Sladarsky, Prague (Czech Republic) | Guido, L.F.,Univesidade do Porto (Portugal). Departamento de Quimica
Ten different barley cultivars and their corresponding malts were analysed for monomeric and oligomeric flavan-3-ols content by using high performance liquid chromatography ultraviolet detection-electrospray ion trap mass spectrometry. The Folin-Ciocalteau and the vanillin spectrophotometric assays were used for the assessment of the total polyphenol and total flavan-3-ol content, respectively. The antioxidant activities of phenolic fractions were investigated using the radical scavenging assay and the ferricyanide reducing power. Catechin and prodelphinidin B3 were respectively the major monomeric and dimeric flavan-3-ols. Moreover, prodelphinidin B3 was shown to be the main contributor for the radical scavenging activity both for barley and malt. The same samples were used to obtain different fractions. The contribution of bound phenolics to the total polyphenol content was significantly higher than that of free and esterified fractions. Catechin and ferulic acid were the most abundant phenolics in the free and bound fractions, respectively. Further, the hot water extracts of barley cultivars and corresponding malts were analyzed. The ferric reducing antioxidant power and radical scavenging activity and indicator time test were compared. The total polyphenol content was positively correlated with all antioxidant methods used. The dominant phenolic compound was ferulic acid.
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