Ascorbic Acid Diminishes Developmental Damages Induced by High Glucose in Mouse Pre-implantation Embryos in vitro
2001
Amiri, I., School of Medicine, Hamedan University of Medical Sciences, Hamedan, Iran | Barbarestani, M., School of Medicine, Tehran University of Medical Sciences, Tehran, Iran | Marzban, H., School of Medicine, Tehran University of Medical Sciences, Tehran, Iran | Nematollahi, N., School of Medicine, Kerman University of Medical Sciences, Kerman, Iran | Niknafs, B., School of Medicine, Tabriz University, Tabriz, Iran
Diabetic teratogenicity seems that to be related to embryonic oxidative stress and the extent of the embryonic damage can apparently be reduced by antioxidants. In this study, 2-cell stage embryos of normal mice were cultured for 96 h with a high concentration of glucose (30 mM) in the absence or presence of 1 mM L-ascorbic acid (LAA). In the end of culture, the morphology of embryos was assessed with the aid of inverted phasecontrast microscope, and also cell apoptosis was studies by TUNEL (TdTmediated conjugated dupt nick end-labeling) technique. Result: High glucosis alone decreased the blastosyst formation and increased the incidence of embryo fragmentation and cell apoptosis. With LAA, the incidence of high glucosis induced embryo fragmentation and cell apoptosis was decreased, on the contrary the incidence of blastocyst formation was increased.
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