Polyethylene glycol improves somatic embryo maturation in big-leaf mahogany (Swietenia macrophylla King, Meliaceae)
2009
Maruyama, T.E., Forestry and Forest Products Research Inst., Tsukuba, Ibaraki (Japan)
The effect of polyethylene glycol (PEG) treatment on somatic embryo maturation in Swietenia macrophylla was investigated. The addition of PEG to the medium stimulated maturation of somatic embryos and subsequently enhanced germination and conversion efficiency. The best results for cotyledonary somatic embryo production, frequency of germination and plantlet conversion were achieved using medium supplemented with 7.5% PEG. Compared with the control (PEG-free treatment), significant enhancement was recorded in the number of cotyledonary embryos harvested (7 to 56), germinated embryos (25 to 59%) and embryos converted into plantlets (5 to 32%). Regenerated plantlets were successfully acclimatized and transferred to a glass-house.
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