Optimization of Solid-State Fermentation Condition Using Distiller's Dried Grain
2008
Choi, G.W. (Changhae Institute of Cassava and Ethanol Research, Changhae Ethanol Co., Ltd., Jeonju, Republic of Korea), E-mail: changrd@chethanol.com | Moon, S.K. (Changhae Institute of Cassava and Ethanol Research, Changhae Ethanol Co., Ltd., Jeonju, Republic of Korea) | Kim, Y. (Changhae Institute of Cassava and Ethanol Research, Changhae Ethanol Co., Ltd., Jeonju, Republic of Korea) | Jang, B.W. (Changhae Institute of Cassava and Ethanol Research, Changhae Ethanol Co., Ltd., Jeonju, Republic of Korea) | Kim, Y.R. (Chonbuk National University, Jeonju, Republic of Korea) | Chung, B.W. (Chonbuk National University, Jeonju, Republic of Korea)
To enhance the value as a feedstuff of distiller's dried grain (DDG) and develop fermented feedstuff, we investigated the effects of the culture conditions affecting glucoamylase activity, such as pH in submerged culture and moisture content in solid-state culture. Also, we investigated the optimal mixing ratio of DDG and wheat bran for the production of fermented feedstuff containing high content of amino acids. In culture conditions for high fermented activity, pH and moisture were optimum at pH 4 and 60%, respectively. In the case of mixing ratio, the glucoamylase activity was decreased with increase of DDG content. On the other hand, the content of crude protein was increased slowly. For the development of fermented feedstuff, the optimal mixing ratio of DDG and wheat bran was 1 to 4. Finally, we could produce approximately 1 ton (dry matter) of trial product in incubator of pilot-scale. The glucoamylase activity and the crude protein content were 1,024 U/g and 33.6%, respectively.
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