Comparative serological analysis of outer membrane proteins extracted from Brucella abortus Korean isolates and 1119-3 strains
2008
Cha, S.B. (Seoul National University, Seoul, Republic of Korea) | Kang, M.L. (Seoul National University, Seoul, Republic of Korea) | Lee, W.J. (Seoul National University, Seoul, Republic of Korea) | Shin, M.K. (Seoul National University, Seoul, Republic of Korea) | Cho, D.H. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea) | Jung, S.C. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea) | Yoo, H.S. (Seoul National University, Seoul, Republic of Korea), E-mail: yoohs@snu.ac.kr
Brucellosis is one of the most important zoonosis in worldwide. As one of the control measures, attempts have been made to develop new diagnostic methods using filed isolates as a national policy in many countries. Currently, bovine brucellosis in Korea have been received attention in both public health and economical aspects due to sudden increase of outbreak. Based on the situation, we compared standard strain (B. abortus 1119-3) with field isolates to reveal the differences among them. Biological and biochemical charateristics, antibiotic resistance profiles, outer membrane proteins (OMPs) and lipopolysaccharide analysis of the strains were included in this study. For the diagnostic purpose, an attempt was made to find out a novel antigen from the Korean isolates by serological analysis. There were differences about 55 kDa, 36-38 kDa and 20 kDa in analysis of OMPs by SDS-PAGE and Western blot with positive sera (greater-than or equal to 1:400 in SAT titer). Also, a serological diagnostic method, ELISA was conducted using OMPs of the strains as novel antigen. Relationships between O.D. and SAT titer were analyzed using field sera showing different SAT titer. High correlation coefficient was observed between SAT titer and ELISA. Results from this study suggested that a new diagnsotic method should be developed using their own field isolates in each country.
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