Technique for making a transparent fish skeletal specimen by using dried food fishes
2010
Konishi, M., Kinki Univ., Nara (Japan). School of Agriculture | Asai, T. | Takeuchi, H. | Hosoya, K.
Possible application of a modified double staining technique to make a transparent skeletal specimen by using dried food fishes is proposed. The steps of the present technique, modified from those of previous techniques based on museum or fresh specimens, are as follows: (1) Soaking of dried fishes for almost a day in water to remove salt before fixation; (2) fixation of specimens in relatively thick formalin; and (3) digestion of muscle with a low-concentration KOH solution. This new technique results in dried fishes more transparent, compared with the results of the conventional techniques.
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