Superfecundation induction by intrauterine insemination with different frozen-thawed canine semen and parentage test using microsatellite analysis
2009
Lee, J.H., Chungnam National University, Daejeon, Republic of Korea | Kim, K.J., Chungnam National University, Daejeon, Republic of Korea | Choi, S.A., Chungnam National University, Daejeon, Republic of Korea | Li, Xiaoxia, Chungnam National University, Daejeon, Republic of Korea | Kim, E.Y., Chungnam National University, Daejeon, Republic of Korea | Oh, H.J., Seoul National University, Seoul, Republic of Korea | Lee, B.C., Seoul National University, Seoul, Republic of Korea | Kim, H.J., Haemaru Small Animal Referral Hospital, Sungnam, Republic of Korea | Park, B.K., Kongju National University, Yesan, Republic of Korea | Kim, M.K., Chungnam National University, Daejeon, Republic of Korea
This study was performed to investigate the possibility of superfecundation by surgical intrauterine artificial insemination in dogs of confirmed genetic pedigree. Artificial insemination was performed on 3 days after ovulation with 1.3×10∨8 spermatozoa. Five puppies were delivered on 60 days after insemination. The ratio of the number of newborns to the number of corpora lutea was 83.3% (5/6). Parentage analysis with 10 canine-specific microstatellite markers demonstrated that one puppy was genetically relative to the sire-A family and four puppies were genetically relative to the sire-B. The present study demonstrated that two kinds of puppies with different genetic pedigree can be produced by surgical uterine insemination of semen of individual dog into each uterine horn of a bitch.
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