In vitro culture of Balkan endemic and rare Pulsatilla species for conservational purposes and secondary metabolites production
2009
Danova, K., Sofia University St. Kliment Ohridski, Sofia (Bulgaria). Department of Plant Physiology | Bertoli, A., Universia di Pisa, Pisa (Italy). Dipartimento di Scienze Farmaceutiche | Pistelli, L., Universia di Pisa, Pisa (Italy). Dipartimento di Biologia delle Piante Agrarie | Dimitrov, D., National Natural History Museum, Sofia (Bulgaria). Plant Section | Pistelli, L., Universia di Pisa, Pisa (Italy). Dipartimento di Scienze Farmaceutiche
Shoot cultures of Pulsatilla montana ssp. balcana, P. halleri ssp. rhodopaea and P. slaviankae were initiated from surface-sterilized seeds of the three species, collected at their natural habitats. Shoot cultures of the three species in Murashige and Skoog culture medium (MS) exhibited a chlorophyll a/b (chl a/b) ratio ranging from 1, 86 to 3, 01. Moreover by using different concentrations of benzyladenine and 3-indole butyric acid the chl a/b ratio differed from species to species, showing a different adaptability of species to different media. The shoot cultures of the three Pulsatilla species were also analyzed as an in vitro source of polyphenolic antioxidant substances so they were screened for total phenolics, flavonoids and anthocyanidins content in three media variants, demonstrating the highest content for these substances in P. montana ssp. balcana and P. halleri ssp. rhodopaea in the growth regulator-free and 6-benzyladenine - supplemented medium, while in medium supplemented with auxin and cytokinin all three species produced commensurable levels of the studied metabolites.
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