Irreversible erythrocyte membrane rupture caused by hyperbaric oxygenation
2010
Belic, B., University of Novi Sad, Novi Sad (Serbia). Faculty of Agriculture | Cincovic, M.R., University of Novi Sad, Novi Sad (Serbia). Faculty of Agriculture | Belic, J. | Vidovic, B.
Hyperbaric oxygen is often used for therapeutic purposes, but also as a model for investigation of oxidative stress of erythrocytes. The aim of the study was to investigate why 40 minutes after exposure to hyperbaric oxygen caused irreversible damage to red blood cells. In trial was attended by 60 mice strain Mus musculus, which are exposed to oxygen under pressure of 3.5 ATA. Blood was taken by vein-puncture and prepared to observe the scanning (SEM) and transmission electron microscopy (TEM). The reason of serious irreversible damage is a red blood cell membrane rupture, which occurs in the area with thin wall membrane. At that point the content of red blood cells pour out in the external environment, and diameter of damage was about 1 microm.
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