Determinarion [i.e. Determination] of Gli-A1 alleles in 13 Osijek [Croatia] cultivars of wheat Triticum aestivum L. | Determinacija Gli-A1 alela kod 13 osijeckih [hrvatskih] sorti psenice Triticum aestivum L.
2010
Knezevic, D., Poljoprivredni fakultet, Zubin Potok (Serbia) | Kovacevic, V., Poljoprivredni fakultet, Osijek (Croatia) | Paunovic, A., Agronomski fakultet, Cacak (Serbia) | Madic, M., Agronomski fakultet, Cacak (Serbia) | Djukic, N., Prirodno-matematicki fakultet, Kragujevac (Serbia)
In this paper, the composition of gliadin controlled by Gli-A1 alleles has been investigated, as well as polymorphism of alleles at Gli-A1 locus in 13 winter wheat cultivars. Electrophoregrams obtained by polyacrylamide gel electrophoresis were used for estimation variability of gliadin components and identification of gliadin blocks. By analysis of 13 wheat cultivars were identified 4 Gli-A1 alleles (a, b, c, m). Variability of determined gliadin block components indicates that existing polymorphisms of gliadins alleles Gli-A1 loci. Frequency of identified 4 alleles at Gli-A1 locus was different and varied from 7.7% (c) to 53.8% (a). Alleles with high frequency could indicate their favourable adaptive and selection value and could be results of limited genetic variability for crossing or direct selection of desirable traits.
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