A successful use of a new shuttle cloning vector pA13 for the cloning of the bacteriocins BacSJ and acidocin 8912
2010
Kojić, M., Institute of Molecular Genetics and Genetic Engineering, Belgrade (Serbia) | Lozo, J., Institute of Molecular Genetics and Genetic Engineering, Belgrade (Serbia) | Jovčić, B., Institute of Molecular Genetics and Genetic Engineering, Belgrade (Serbia) | Strahinić, I., Institute of Molecular Genetics and Genetic Engineering, Belgrade (Serbia) | Fira, D., Institute of Molecular Genetics and Genetic Engineering, Belgrade (Serbia) | Topisirović, L., Institute of Molecular Genetics and Genetic Engineering, Belgrade (Serbia)
The aim of this paper was to research the molecular cloning of genes encoding the novel bacteriocin BacSJ from Lactobacillus paracasei subsp. paracasei BGSJ2-8 by using a newly constructed shuttle cloning vector pA13. A new shuttle cloning vector, pA13, was constructed and successfully introduced into Escherichia coli, Lactobacillus and Lactococcus strains, showing a high segregational and structural stability in all three hosts. In conclusion, the strain L. paracasei subsp. paracasei BGSJ2-8 was isolated from home made semi-hard cheese manufactured in a village household. BGSJ2-8 produces two bacteriocins, designated BacSJ and acidocin 8912. To our knowledge, BacSJ is the first completely characterized bacteriocin produced by the L. paracasei subsp. paracasei species. The genes encoding for bacteriocin BacSJ and acidocin 8912 production and immunity are located on the 14.4 kb large plasmid pSJ2-8. The molecular characterization of the plasmid pSJ2-8 was performed using the new shuttle cloning vector pA13.
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