Establishement [i.e. Establishment] of aseptic culture in vitro for new vegetative rootstocks for cherry, pear and plum | Uspostavljanje aseptične kulture in vitro novih vegetativnih podloga za trešnju, krušku i šljivu
2010
Ružić, Đ., Institut za voćarstvo, Čačak (Serbia) | Cerović, R., Institut za voćarstvo, Čačak (Serbia) | Vujović, T., Institut za voćarstvo, Čačak (Serbia)
Explant sterilization procedure, the initial phase of micropropagation, is very important for the successful organogenesis outcome, especially when initial explants are collected from open field (a planting) or glass house/screen house. Standard procedure of surface sterilization, modified in terms of time, has been applied on Gizela, Pyrodward and Fereley Jaspi rootstocks. Two types of explants were used: buds taken from twigs maintained in laboratory, and from those maintained in a screen house. The highest rate (%) of induced leaf rosettes was obtained from laboratory explants of Gizela 5 (82.7%). Besides type and method of taking explants, which are major factors of successful establishment of sterile culture, the method of surface sterilization, modified in terms of time, has also proved a significant segment. In the procedure of establishment of aseptic culture experience plays an important role. It varies among laboratories, as each micropropagation laboratory introduces new modifications. Nevertheless, any innovation represents a very important contribution to the success of this modern method of plant/fruit propagation which can be widely applied, particularly in commercial laboratories.
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