Features of relations parasite-host at experimental infection of laboratory rodents by arctic isolate Trichinella nativa | Особенности паразито-хозяинных отношений при экспериментальном заражении лабораторных грызунов арктическими изолятами Trichinella nativa

Biological properties of arctic isolates Trichinella nativa allocated of muscles of polar bear and glutton lived in different zoogeographical biocenosis of Yakutia are investigated. Adaptive reactions, immunifacient properties and strain distinctions of T. nativa are investigated at passaging on laboratory rodents. The comparative analysis of productivity passaging and screening of proteolytic properties in relation to 16 synthetic substrata researched arctic isolates of T. nativa is carried. It is shown that at freezing at - 18 deg C larva taking place in muscles of polar bear and glutton during 26 months, the significant part of helminths has remained viable. In muscles of white rats resistance of T. nativa to freezing is sharply reduced, by 5 day keep viability less than 10% of parasites, by 7 day all larva perish. Resistance of T. nativa to sharp downturn of temperature is genetically caused attribute which depends on degree of adaptation to concrete organism of the animal-host. ELISA of blood serum of laboratory animals inoculated with Trichinella of polar bear has shown that specific antibodies in minimum diagnostic titer were recorded at day 30 of invasion and reached maximum by day 90 retaining in blood in titers 1:320 and 1:1640 to the end of experiment. A comparative analysis of spectroproteolytic activity of invaded larvae showed similarity of the spectrum of proteolytic enzymes of the two artic isolates of Trichinella caused by the belonging to the same genotype – T. native and difference in the quality of proteases in excretory and secretory products. An analysis of phenotypic variability of Trichinella isolates has found the great differences of artic isolates in the degree of adaptation, freezing resistance, response of animals to capsule forming of Trichinella and the number of proteolytic enzymes.