Enzyme-linked immunosorbent assay of chloramphenicol in foodstuff | Иммуноферментный анализ хлорамфеникола в продуктах питания
2009
Fedorova, M.D. | Rubtsova, M.Yu. | Samsonova, Zh.V. | Egorov, A.M., Lomonosov Moscow State Univ. (Russian Federation) | Vylegzhanina, E.S. | Komarov, A.A., All-Russia State Centre of Quality and Standardization of Drugs for Animals and Feeds, Moscow (Russian Federation) | Andreeva, I.P.
A test-system based on enzyme-linked immunosorbent assay (EL1SA) for the quantitative detection of chloramphenicol (CAP) in foodstuff has been developed. The detection limit of the method was 0,05 u.g/1. The procedures for milk samples preparation of various fat content and chicken muscles were optimized. Before the analysis, milk was 5-fold diluted with a buffer. The detection limit for milk was 0,3 u.g/1; recoveries varied from 74 to 118%. Two protocols for chicken muscles preparation were elaborated, extraction with buffer (express method) and extraction with acetonitrile. The detection limits of CAP in chicken muscles were 0,5 u.g/kg and 0,3 ng/kg, respectively; recovery values were 71—107% and 95—115%, respectively. The results of the CAP residues detection in foodstuff by ELISA and HPLC-MS were in good correlation
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