Устойчивость к бактериозам генетически модифицированных растений картофеля
2009
Polityko, V.A. | Kornev, K.P., All-Russia Research and Development Inst. of Phytopathology, Moscow Region (Russian Federation) | Kirsanova, S.N., The A.G. Lorkh All-Russia Research and Development Inst. of Potato Production, Moscow Region (Russian Federation) | Karlov, A.N., Russian State Agrarian Univ., Moscow (Russian Federation) | Kochetov, A.V. | Kolodyazhnaya, Ya.S., Russian Academy of Sciences. Siberian Branch, Novosibirsk (Russian Federation). Institute of Cytology and Genetics | Ignatov, A.I.
The experiments involved genetically modified potato plants of Nikulinsky variety carrying gene of Arabidopsis proline dehydrogenase arranged in antisense orientation. They were studied for resistance to some bacterial pathogens (strains of Clavibacter, Erwinia, Pseudomonas genera) – causing agents of different bacterial blights, brown rot, bacterial wilts. The potato variety Zhoukovsky susceptible of bacterial pathogens was the control. The infestation of plants was carried out by different methods to estimate the resistance: immersing sprout tops into a bacterial suspension, infection to a stem, infesting separate leaves. Tubers obtained from meristems plants were also incubated by several methods. No symptoms of disease were found in the variants of weakened infestation with soft rot agents in genetically modified potato plants, whereas in the control variant they were present. When infesting with specialized pathogens of clavibacteria group and brown rot agents the advantage of the genetically modified potato plants exhibited only in some combinations plant-pathogen. It is concluded that the increased content of proline in plant cells increases their resistance to bacterial blights in stress conditions
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