The results of phytoplasms testing in stone fruits (Prunus spp.) in Slovenia ( 2000-2006) | Pregled rezultatov dolocanja fitoplazem na koscicarjih v letih 2000-2006 v Sloveniji
2007
Mehle, N., National Inst. of Biology, Ljubljana (Slovenia) | Brzin, J. | Boben, J. | Hren, M. | Frank, J. | Petrovic, N. | Gruden, K. | Dreo, T. | Zezlina, I. | Seljak, G. | Ravnikar, M., National Inst. of Biology, Ljubljana (Slovenia)
Phytoplasms are small wall-less prokaryotes that live exclusively in sieve tubes of their host plants, and cause many important vector-borne and graft-transmissible plant diseases. In Europe, stone fruits are seriously affected by the European stone fruit yellows (ESFY) phytoplasma ('Candidatus Phytoplasma prunorum'), belonging to the group 16SrX. In years 2000-2006 more than 400 stone fruits sampled in different regions of Slovenia were tested for ESFY. The majority of the sampling was done in frame of a survey of the presence of ESFY in the country, and samples were taken from the trees with and without expressed symptoms of ESFY. In addition, wilting and dying cherries (Prunus avium) from Western Slovenia were sampled. Different extraction methods from the roots and asymptomatic shoots were compared. All samples were tested using methods of nested PCR, RFLP, and sequencing. The phytoplasms were confirmed in Prunus persica, P. armeniaca, P. salicina, P. domestica, P. avium, P. cerasifera and Caccopsylla pruni.
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