Determination of isoflavone from soybean lines cultivated in Jilin Province and correlation analysis between isoflavone content and soybean quality | 吉林省栽培大豆品系异黄酮含量测定及其与大豆品质的相关性分析
2010
Song Xiangdong, Jilin Academy of Agricultural Sciences, Changchun (China) | Zhang Zhixin, Jilin Academy of Agricultural Sciences, Changchun (China) | Wang Weiwei, Jilin Academy of Agricultural Sciences, Changchun (China)
[Objective] The aim of this study was to set up a high performance liquid chromatography for rapid determination of isoflavones from soybean and analyze the correlation between isofalvone content and protein or fat content. [Method] The isoflavones were firstly extracted by 80% methanol and then hydrolyzed at 100 ℃. The chromatographic separation adopted a reversed-phase C18 analytical column with binary high-pressure gradient elution, while its analysis time was 25 min and column temperature was 40 ℃. The diode array detector was used for monitoring with wavelength of 260 nm. The correlation between isofalvone content and protein or fat content was analyzed by data processing system Origin 6.0. [Result] The high performance liquid chromatograph for determination of isoflavones from soybean was verified to be accurate and reliable by methodology. The isoflavones of 85 soybean lines cultivated in Jilin Province was determined, and the results primarily showed the characters and ranges of isoflavones from soybean lines cultivated in Jilin Province, while the isoflavone content of soybeans ranged from 2.29 to 4.89 mg/g, and the average content was 3.41 mg/g. The isoflavone content of 5 soybean lines exceeded 4 mg/g, while there was a remarkably negative correlation between isoflavone content and protein content, and there was no significant positive correlation between isoflavone content and fat content. [Conclusion] The isoflavone content of soybean lines cultivated in Jilin Province is higher, so it is feasible for breeding the soybean lines with high isoflavone content and fat contetnt.
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