Isolating and screening of effective decomposing strain of silkworm chrysalis protein and study on parts of its enzymatic properties | 蚕蛹蛋白高效分解菌的分离筛选及部分酶学性质的研究
2010
Kong Jun, Guangdong Academy of Agricultural Sciences, Guangzhou (China), Institute of Agricultural Sciences | Tu Guoquan, Jiangxi Agricultural University, Nanchang (China), College of Bioscience and Engineering | Kuang Zheshi, Guangdong Academy of Agricultural Sciences, Guangzhou (China), Institute of Agricultural Sciences
[Objective] The research aimed to obtain an effectively-decomposing strain of silkworm chrysalis protein and discuss its enzymatic properties. [Method] The effectively-decomposing bacteria of protein was isolated from the decayed silkworm chrysalis by using dilution plate and its enzymatic properties were tested after primary screening and second screening. The enzyme activity was determined and the intermediate and small molecule protein content in silkworm chrysalis was measured after solid-state fermentation. [Result] An effectively-decomposing strain of silkworm chrysalis protein was obtained and its optimum pH value and temperature were 8.0 and 55 ℃ respectively. The content of intermediate and small molecule protein of silkworm chrysalis powder reached 15.09% after fermentation, 95.72% higher than control without fermentation. [Conclusion] The effectively-decomposing bacteria of silkworm chrysalis protein was preliminarily obtained.
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