SSR analysis of three species from primary parent and their first generation of Litopenaeus vannamei | 凡纳滨对虾3个亲本及其子代群体的SSR分析
2010
Li Zhimin, Guangdong Ocean University, Zhanjiang（China）, Fisheries College | Xie Li, Guangdong Ocean University, Zhanjiang（China）, Fisheries College | Ye Fuliang, Guangdong Ocean University, Zhanjiang（China）, Fisheries College
Genetic variations of three species from primary parent（OI Q，SIS Q，Kona Bay Q）and their first filial generation（OI Z，SIS Z，Kona Bay Z）of Litopenaeus vannamei were detected using microsatellite marker technique. The amplified products of genomic DNA with 8 microsatellite makers indicated 8 loci presents polymorphism，the number of total alleles were 28 in 6 species；the number of allele every loci was 2 to 6；mean allele number（Na）was 3.5；expected heterozygosity value（He）were higher than observed heterozygosity value（Ho）；mean polymorphism information content（PIC）ranged from 0.479 4 to 0.769 9，which showed these 8 loci posses relatively high information content. Through analysis of genetic structure in primary parent and their first filial，allele and effective allele number，observed heterozygosity and expected heterozygosity values and mean polymorphism information content（PIC）in filial generation were little lower than their primary parents. However，genetic diversity of filial generation is not influenced，and still maintains a good heritability.
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