Research on pollen grains of tetraploid lines and diploid control line of Chrysanthemum cinerariifolium (Trev.) Vis. | 白花除虫菊四倍体与二倍体株系花粉粒的比较研究
2010
Liu Zhen, Southwest Jiaotong University, Chengdu (China) | Gao Shanlin, China Pharmaceutical University, Nanjing (China)
[Objective] The aim was to study characters of pollen grains of tetraploid lines and diploid control line of Chrysanthemum cinerariifolium (Trev.) Vis., morphological characters, fertility of pollen grain and germination percentage of seeds. [Method] Pollen grains were prepared by sulphuric acid-acetyl oxide decomposition method. The lengths of polar axis and equatorial axis of pollen grains were determined with general optical microscope. The morphology of pollen grains was observed with SEM (scanning electron microscope) and the typical visual fields of 2 500× (or 2 000×), 7 000× were taken pictures. [Result] Comparing with the diploid control line, the pollen grains of five tetraploid lines which were tested were different from the diploid line in morphology, sculpture, etc.. Four of the five tested samples were significant larger than the diploid line in size and one was similar to the diploid line. [Conclusion] This research provided references for breeding tetraploid improved varieties of Chrysanthemum cinerariifolium (Trev.) Vis. with good fertility and high germination percentage.
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